GENETIC DIVERSITY OF EXON 2 OF OVINE MAJOR HISTOCOMPATIBILITY COMPLEX OF (MHC) CLASS 11 DRB1GENE IN SOME NIGERIAN SHEEP BREEDS
DOI:
https://doi.org/10.33003/jaat.2024.1002.09Keywords:
Sheep breed,, DRB1 Gene,, PCR-RFLP,, Major Histocompatibility Complex, and Genetic diversityAbstract
The genetic diversity of three Nigerian sheep of breeds was investigated based on the DRBI (exon2) an immune gene. Blood samples from 150 sheep (50 each from Balami, Uda, and Yankasa breeds) were collected from Maiduguri Abattoir. The samples were analyzed at the Biotechnology Centre of the University of Maiduguri. The analysis is based on PCR-RFLP; the genomic DNA of sheep was carried out from whole blood sample using DNA extraction kit to extraction DNA. PCR product was incubated and digested using the restriction enzyme Rsa1. The analysis of molecular variance (AMOVA) shows that only 11 percent of the total variations were observed in the sampled population and accounted for population differences, and 89 percent was accounted for within populations. The phylogenetic tree showed that Uda and Yankasa are closely related to each other than the Balami sheep.
References
Adamu, S. B., Muhammad, A. R. and Aliyu, J. (2008). Appropriate Switch-Over Age from Broiler Starter to Broiler Finisher Diet Under a Hot Dry Environmental Condition. Jolorn 9 (2): Pp. 173-179.
Adebambo, O., Williams J. L, Bloot, S. and Urquhhart B. (2004). Genetic relationships between native sheep breed of Nigeria based on microsattelite DNA polymorphism. Animal genetic resources information, 34, 27-40.
Adebambo, O., Williams, J. I., Blott, S. and Urquhart, B. (1999). Genetic relationships between native sheep breed in Nigeria based on microsatellite DNA polymorphisms. Nigerian journal of Animal production 27 (1):1-8
Agaviezor, B. O., Adefenwa. M.A., Peters, S. O., Yakubu. A., Adebambo, O. A., Ozoje. M.O., Ikeobi, C. O. N., Ilori, B. M., Wheto, M., Ajayi, O. O., Amusan, S. A., Okpeku, M., De Donato, M. and Imumorin, I. G. (2012). Genetic diversity analysis of the mitochondrial D-loop 0f Nigerian indigenous sheep Animal Genetic resources 50:13-20
Akinyemi, M. O., and Salako, A. E. (2012). Genetic relationship among Nigerian indigenous sheep population using. blood protein polymorphism. Agricultural science and technology, 4(2):107-112.
Alaku, S. O. (2011). Introduction to animal science published by JEE Communication J Ogui road, Enugu. pp. 158-162.
Aliyu, J. (2013). Productivity Assessment of Four Strains of Indigenous Chicken in a Semi-Arid Region of North-Eastern Nigeria. Ph.D. Thesis, Department of Animal Science, University of Maiduguri, Maiduguri, Nigeria.
Boujenane, I., Ouragh, L., Benlamlih, S., Aarab, B., Miftah, J. and Oumrhar, H. (2008). Variation at post-albumin, transferrin and haemoglobin proteins in Moroccan local sheep. Small Ruminant Research 76, 113-117.
Daryl, I. K. and Steve, B. N. (2001). Application of selection concepts for genetic improvement. Purdue University Cooperative Extension Services, West Lafayette,IN47907. Fact sheet Number 9.
Dauda, A., Abbaya, H. Y., Shettima, M. S., Sinodo, S., Malgwi, I. H and Abare, E. A. (2016). Sequence length variation and Transmembrane Analysis of Contagious Bovine Pleuro pneumonia (CBPP) and Contagious Caprine Pleuro pneumonia (CCPP) Proteins. Journal of Veterinary and Animal Research. 4(3):121-127.
FAOSTAT. (2022). Food and Agriculture Organization of the United Nations. Statistical Database. Rome, Italy.
Floudas, C. A. (2007). Computation methods in protein structure prediction. Biotechnology and Bioengineering, 97(2):207-213.
Gibson., J. P and Bishop, S. (2005). Use of molecular markers to enhance resistance of livestock to disease: a global approach. Rev.sci. tech. off. Int. Epiz., 24(1)
Gizaw, S., Komen, H., Hanote, O., Van Arendonk, J. A. M., Kemp, S., Aynalen, H., Mwai, O. and Dessie, T. (2011). Characterization and conservation of indigenous sheep genetic resources: A practical framework for developing countries. ILRI Research Report 27. Nairobi, Kenya.
Green, M. and Sambrook, J. (2012) Molecular Cloning: A Laboratory Manual. 4th Edition, Vol. II, Cold Spring Harbor Laboratory Press, New York.
Mohammed, A., Raji, A.O. and Igwebuike, J.U. (2018). Genetic variability of Nigerian sheep breeds at the insulin-like growth factor-1 (IGF-1) gene locus. Nigerian Journal of Animal Production, 45(1):51-60.
Mwacharo, J. M., Otieno, C. J. and Okeyo, A. M. (2002). Genetic variations between indigenous fat-tailed sheep populations in Kenya. Small Ruminant Research, Volume 44,173-178.
Nei, M. (1987). Estimate of average of average heterozygosity and genetic distance from all small number of individuals. Genetics, 89:583-590.
Ordas, J. G. and Primitivo, F. S. (1986). Hemotypes in Spanish sheep breeds, Comparative Biochemistry and physiology part A. Physiology. 85:(1) 73-76.
Pariset, L., Mariotti, M., Gargani, M., Joost S., Negrime, R., Perez, T., Bruford, M., Marsan, P.A. and Valentini, A. (2011). Genetic diversity of sheep breeds from Albania, Greece, and Italia assessed by Mitochondrial DNA and nuclear polymorphism (SNPs). The Scientific World Journal, 11:1641-1659.
Raji, A. O., Igwebuike, J. U. and Kwari, I. D. (2010). Regression models for estimating breast, thigh and fat weight and yield of broilers from non-invasive body measurements. Agric. Biol. J. N. Am. 1(4):469-475.
Sun, X., Henderson, G., Cox, F., Molano, G., Harrison, S. J., Luo, D., Janssen, P. H., Pachaco, D. (2015). Lambs fed fresh winter forage rape (Brassica napusL) emit less methane than those fed perennial ryegrass (Lolium perenne L.) and possible mechanisms behind the difference. PLOS Journal, 10(3):e0119697.
Wang, Y. E. and Jang, H. (2003). Cloning and characterization of Bovine class and class two insulin –like growth Factor mRNAs. Domestic Animal Endocrinology 25.31-328.
Yunusa, A. J., Salako, A. E. and Oladejo, O. E. (2013). Morphometric characterization of Nigerian indigenous sheep using multifactorial discriminant analysis. International Journal of Biodiversity and conservation.5 (10):661-665.
Zanotti, C. M, Gandimi, G. C. and Leon, P. (1990). Genetic variation and distances of five Italian native sheep breeds. Animal Genetics, 21, 87-92.
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